un-matched healthy samples and tumor fraction calculation

[frasca17]

Dear Amjad Alkodsi, I am a bioinformatician, and I was wondering if is it possible to use un-matched healthy samples respect to the tumor samples that we sequenced. We cannot sequence normal tissue, so we were thinking of using some neonatal bam files taken online to compare our samples.

We are actually interested in calculating the tumor fraction of these samples, so If you have also suggestions, they are welcome.

Thank you very much for your availability