fix: CLSM get_fluorescence_decay and get_phasor #49
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…ecision loss in get_phasor - get_fluorescence_decay: when stack_frames=true, now correctly processes all frames instead of only the first frame. Uses target_frame index to write all photons to frame 0 of output while iterating over all n_frames. - get_phasor: fix precision loss in non-stacked branch where phasor values (floats between -1 and 1) were cast to int, truncating all precision. Changed static_cast<int> to static_cast<float>. - Update test reference files to reflect correct stacked decay behavior.
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cqian89
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…y for irregular frames
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Pull request overview
This pull request fixes two bugs in CLSM (Confocal Laser Scanning Microscopy) analysis methods related to frame stacking and numerical precision.
Changes:
- Fixed
get_fluorescence_decayto correctly accumulate data from all frames whenstack_frames=True, instead of only processing the first frame - Improved numerical precision in
get_phasorby casting tofloatinstead ofint, preventing truncation of decimal values - Updated test reference data to reflect the corrected output
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Thanks for the PR and the detailed description and validation against the BH vendor exports. I am currently planning a new release and will integrate your changes as part of that effort. The upcoming release focuses mainly on improved memory management (no API changes) and is a slightly larger update; current ETA is end of February. I will keep the pull request open for now, integrate your changes internally, and then merge your PR as part of the next release. Afterwards, I will take care of publishing the update to conda-forge and pip (on my personal TODO list). Thanks again for the careful implementation! |
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Summary
get_fluorescence_decayto correctly handlestack_frames=Trueby accumulating data from all frames into the first output frame.get_phasorby usingfloatinstead ofintfor result storage.