Python-based GUI application for laboratory calculations (DNA analysis, Tm calculation, and molarity)

BioTech Lab Assistant (GUI)

BioTech OS Pro

Core Modules

• Sequence Analysis: DNA/RNA to Protein translation with ORF finder.
• Restriction Mapping: Virtual digestion with common enzymes (EcoRI, HindIII, etc.).
• Protein Biophysics: Molecular weight, Hydropathy profile, and Isoelectric Point (pI) calculation.
• Alignment Engine: Pairwise sequence alignment using the Needleman-Wunsch algorithm.
• Genome Stats: GC-skew analysis and nucleotide distribution tracking.

A desktop application designed to streamline routine laboratory calculations for biotechnologists. This tool provides a user-friendly interface for DNA analysis and PCR primer preparation.

Click to expand gallery

Main Dashboard

Sequence Statistics

Alignment & Visualization

Current Features

• Tm Calculation: Estimates the melting temperature (Tm) of oligonucleotide primers using the Wallace Rule (Salt-adjusted formula for sequences <14-20 bp).
• GC-Content Analysis: Calculates the percentage of Guanine and Cytosine to predict primer stability and secondary structures.
• Input Validation: Built-in error handling to ensure only valid genomic data (A, T, G, C) is processed.

Scientific Background

The melting temperature is calculated based on the following thermodynamic approximation: $Tm = 2(A+T) + 4(G+C)$

This is a fundamental calculation for determining the optimal annealing temperature in PCR protocols.

Technology Stack

• Language: Python 3.x
• Library: Tkinter (Standard GUI Library)

Future Updates

• Molarity and dilution calculator.
• Reverse complement sequence generator.
• Protein molecular weight estimator.

How to Run

1. Ensure you have Python installed.
2. Download dna_calculator.py.
3. Run the script: python dna_calculator.py A comprehensive Bioinformatics Analysis Environment built with Streamlit and Python.